Ученые Института гидробиологии и экологии изучают морфологическое разнообразие и закономерности роста отолитов рыб для оценки питания каспийского тюленя (Pusa caspica) в периоды залегания на лежбищах.
Influenza viral vectors expressing the Brucella OMP16 or L7/L12 proteins as vaccines against B. abortus infection
Автор(ы): Tabynov K. K.*Sansyzbay A. R.*Kydyrbayev Zh. K.*Yespembetov B. A.*Ryskeldinova Sh. Zh.*Zinina N. N.*Assanzhanova N. N.*Sultankulova K.*Sandybayev N. T.*Khairullin B. M.*Kuznetsova I.*Ferko B.*Egorov A.*
Реферат: We generated novel, effective candidate vaccine against Brucella abortus based on recombinant influenza viruses expressing the Brucella ribosomal protein L7/L12 or outer membrane protein (Omp)-16 from the NS1 open reading frame. The main purpose of this work was to evaluate the safety, immunogenicity and protectiveness of vaccine candidate in laboratory animals.
Optimization of Equine Influenza Strain A/equine/Otar/764/07 (H3N8) Cultivation in MDCK Cell Culture
Автор(ы): Ershebulov Z. D.*Taranov D. S.*Abduraimov Ye.*Barakbayev K. B.*Kondibaeva Zh. B.*Kaysenov D. N.*Zaynettinova D. B.*Amanova Zh. T.*Sansyzbay A. R.*Sarsenbayeva G. J.*
Реферат: The paper presents the results of a study of cultural characteristics of the strain A/equine/Otar/764/07 (H3N8) of equine influenza virus isolated from sick horse during the outbreak of equine influenza epizooty in the Republic of Kazakhstan. The studies determinedthe optimal culture conditions for equine influenza virus strain A/equine/Otar/764/07 (H3N8) in MDCK cell culture. The optimal infecting dose of the virus was 0,1 TCE50/cell, temperature and cultivation duration - 34 + 0,5 әC, 72 h, respectively, and Trypsin concentration in medium - 2 mg/ml. Subject to the determined parameters of A/equine/Otar/764/07 (H3N8) strain cultivation in MDCK the virus - containing suspension with an infectious titer of at least 7,00 lgTCE50/ml and hemagglutinating titer of 8,0 log2 can be obtained. The results obtained during the strain reproductive characteristics study can be used for the development of vaccines and diagnostic kits.
Studying on pasteurellosis agent isolate from died saiga antelope
Автор(ы): Koshemetov Zh.*Sandybayev N. T.*Sansyzbay A. R.*Orynbayev M. B.*Khairullin B. M.*Nurabayev S. S.*Matveeva V. M.*Bogdanova M.*Sugirbaeva G.*Seisenbayeva M.*Burabaev A.*Yessirkepov M.*
Объем документа: С. 400-407
МРНТИ: 34.27.29*
Ключевые слова: сайгаки*штаммы пастереллеза*
Реферат: The article contains the biological characterization of pasteurellosis strains isolated from dead saiga antelopes in Kazakhstan. Results demonstrated that pasteurellosis strains isolated from saiga antelopes from different periods had identical biological characteristics.
Peste des petits ruminants: Monitoring, diagnostic and spread on the territory of the Central Asia
Автор(ы): Koshemetov Zh.*Sansyzbay A. R.*Sandybayev N. T.*Abduraimov Ye. O.*Matveeva V. M.*Nurabayev S.*Bogdanova M.*Sugirbaeva G.*Burabaev A.*Yessirkepov M.*
Реферат: The monitoring researches and laboratory confirmation of peste des petits ruminants on the territory of the Central Asia is carried out. As a result of the conducted laboratory researches of the delivered biomaterials from sick, died goats and sheep from various regions of the Republic of Kazakhstan, Tajikistan and Kyrgyzstan it has been demonstrated that virus of this disease is present. The map of spread of peste des petits ruminants on the territory of the Central Asia is made.
Optimization of New Recombinant Influenza Virus Expressing M. Tuberculosis Esat6 2a Ag85a Proteins Cultivation in Vero Cells
Автор(ы): T4049, Lot10D169, * Lot020M7022T1426-1g) at a concentration 2 mkg/ml. The TB-FLU Esat6 2A Ag85A recombinant strain expressing mycobacterial proteins Esat6 and Ag85A with open reading frame of NS1 protein of the influenza virus is genetically stable and capable to maintain enthetic inserts during 10 consecutive passages in Vero cell culture. Subject to the established cultivation parameters the recombinant virus accumulation level reached up to 7,75 + 0,08 lg TCD50/cm3 with 7,0 log2hemagglutinating titer, which is quite suitable for the development of a TB vaccine for human health care.*Kondibaeva Zh. B.*Yershebulov Z. D.*Sultankulova K. T.*Nakhanov A.*Taranov D. S.*Bulatov Ye. A.*Khairullin B. M.*Sandybayev N. T.*Sansyzbay A. R.*Koshemetov Zh.*
Реферат: This paper presents the results of the development of the optimal conditions for new TB-FLU Esat6 2A Ag85A recombinant strain cultivation in Vero cell culture. The studies revealed that the maximum accumulation of the virus in cell culture can be achieved with 0,001 TCD50/cell infecting dose, 34,0 + 0,5 oC incubation temperature and 72 hours of incubation time. The highest reproductive activity of the virus was obtained using trypsin (Sigma
Study of Cultural Characteristics and Interference of Peste Des Petites Ruminants Virus and Sheep Pox Virus in Co-Culture
Автор(ы): Taranov D. S.*Yershebulov Z. D.*Amanova Zh.*Bulatov Ye. A.*Barakbayev K.*Abduraimov Ye.*Kondibaeva Zh. B.*Zhugunissov K.*Sansyzbay A. R.*
Объем документа: С. 227-231
МРНТИ: 34.27.19*
Ключевые слова: культивирование штамма*чума мелких животных*вирус оспы овец*культура клеток*
Реферат: The paper presents cultural characterization of Peste Des Petites Ruminants (PPR) virus and sheep pox virus in co-culture. During the study we selected optimal cell cultures such as lamb kidney cells (LK), lamb testicles cells (LT) and goat gonad cell line (CG-91) allowing to obtain virus suspension with high infective activity. The optimum period of cultivation is 7 days and the infecting dose of PPR and sheep pox viruses is 0,01 TCID50/cell. The interference phenomenon absence between the two viruses is confirmed by electron microscopic studies, as well as the cytopathic effect of these viruses in cell culture. Subject to the above parameters highly active virus-containing suspension can be obtained for PPR and sheep pox associated vaccine production.
Comparative Phylogenetic Analysis of the Camelpox Virus Isolated in the Mangistau Region of the Republic of Kazakhstan
Автор(ы): Bulatov Ye. A.*Strochkov V. M.*Zhilin Ye.*Barakbayev K.*Sansyzbay A. R.*
Реферат: The article presents the results of phylogenetic analysis of nucleotide sequences of Camelpox virus strain M-96 isolated in the Mangistau region, Republic of Kazakhstan. The nucleotide sequences of 7 genes of twenty-six CPV strains / isolates were selected from the international GenBank database to determine the degree of M-96 strain nucleotide sequences homology with the other CMLV strains using phylogenetic analysis. As a result of the conducted comparative phylogenetic analysis we determined genetic differences and the degree of Kazakhstan strain homology with other CMLV strains, isolated at different times and in different geographical regions and the ways of possible CMLV introduction on the territory of the Republic of Kazakhstan. Kazakhstan strain M-96 of Camelpox virus showed 100% homology with five genes of CMS strain (Iran, 1970), two genes of SP-1_Iran strain and one gene of Bikaner strain, isolated in 2008 in India. The obtained data analysis suggests that Camelpox virus can be transmitted to the territory of the Republic of Kazakhstan from Iran via Turkmenistan.
Evaluate the immunogenicity antibrucellar vaccine vector model of guinea pigs
Автор(ы): Yespembetov B. A.*Syrym N. S.*Zinina N. N.*Tabynov K. K.*Nissanova R. K.*Sarmykova M. K.*Bezrukova S. Z.*Konbaeva G. M.*
Объем документа: С. 565-568
МРНТИ: 34.25.23*
Ключевые слова: бруцеллез*иммуногенность вакцины*вакцина B. abortus 19*
Реферат: As a result of extensive research, the highest immunological parameters were obtained in the groups of animals immunized with the mixture of designs NS1-124-L7L12 + NS1-124-Omp 16 (H5N1 and H1N1), not inferior piece of the vaccine. B. Abortus 19, which will be recommended for testing in cattle. In determining the immunogenicity of the vaccine constructs selected the most suitable candidate for further research on cattle, because in this group of relatively low II 4.8 and 60% protection.
Kazakhstan under the conditions of the United Economic Area formation (UEA)
Реферат: Under the condition of forcing industrial and innovative processes and creation of UEA there is an objective need to develop scenarios for the competitiveness of uniting the key strategic provisions of programs of modernization of economy of Belarus, Kazakhstan and Russia. Changing the structure of the economy becomes an obligatory condition to overcome the negative structural trends, especially in the fuel and energy and food complex, but as above-mentioned, in the absence of energy and food export-import policy at the national and regional level, it is not possible to perform the objectives of the forced industrialization and the regulation of general energy and food markets in the UEA countries.
RYR2 sequencing reveals novel missense mutations in a Kazakh idiopathic ventricular tachycardia study cohort
Реферат: Channelopathies, caused by disturbed potassium or calcium ion management in cardiac myocytes are a major cause of heart failure and sudden cardiac death worldwide. The human ryanodine receptor 2 (RYR2) is one of the key players tightly regulating calcium efflux from the sarcoplasmic reticulum to the cytosol and found frequently mutated (<60%) in context of catecholaminergic polymorphic ventricular tachycardia (CPVT1). We tested 35 Kazakhstani patients with episodes of ventricular arrhythmia, two of those with classical CPVT characteristics and 33 patients with monomorphic idiopathic ventricular arrhythmia, for variants in the hot-spot regions of the RYR2 gene. This approach revealed two variants; one de-novo RYR2 mutation (c13892A>T; p.D4631V) in a CPVT patient and a novel rare variant (c5428G>C; p.V1810L) of uncertain significance in a patient with VT of idiopathic origin which we suggest represents a low-penetrance or susceptibility variant. In addition we identified a known variant previously associated with arrhythmogenic right ventricular dysplasia type2 (ARVD2). Combining sets of prediction scores and reference databases appeared fundamental to predict the pathogenic potential of novel and rare missense variants in populations where genotype data are rare.
